Part:BBa_K4345017

pSynSense2.5000 PfdeAR-FdeR biosensor circuit

Biosensor circuit in pSynSense2.5000 developed by De Paepe et al. (2018) is composed of TT3-rrnD1-T1, fdeR, rrnB T1 terminator, PFdeAR, and pSynSens2.5000 RBS. The modified LysR-type PfdeAR-FdeR pair from Herbaspirillum seropedicae allows this circuit to respond to naringenin. There are two modules in the circuit: detector and effector. In the detector module, FdeR will produce the transcription factor of the effector module. This transcription factor will bind to the binding site upon the presence of naringenin, thus making this circuit naringenin responsive.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 580
  Illegal EcoRI site found at 1058
  Illegal XbaI site found at 1088
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 580
  Illegal EcoRI site found at 1058
  Illegal NheI site found at 1523
  Illegal NotI site found at 1094
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 580
  Illegal EcoRI site found at 1058
  Illegal BamHI site found at 1073
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 580
  Illegal EcoRI site found at 1058
  Illegal XbaI site found at 1088
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 580
  Illegal EcoRI site found at 1058
  Illegal XbaI site found at 1088
  Illegal NgoMIV site found at 767
  Illegal NgoMIV site found at 779
  Illegal NgoMIV site found at 870
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1544
  

Usage and Biology

This part is part of the biosensor circuit shown below.

Image obtained from De Paepe et. al. (2018)

References

De Paepe, B., Maertens, J., Vanholme, B., & De Mey, M. (2018, April 24). Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor. ACS Synthetic Biology, 7(5), 1303–1314. https://doi.org/10.1021/acssynbio.7b00419